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KMID : 0894520030070020119
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Development & Reproduction
2003 Volume.7 No. 2 p.119 ~ p.126
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Studies on Mammalian Homolog and Flanking Sequence of Mouse MT Transposon-like Element, Clone MTi7(MTi7)
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김영훈/Kim YH
고민수/우대균/최돈찬/이경아/Ko MS/Woo DG/Choi DC/ Lee KA
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Abstract
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In the previous study, we obtained list of differentially expressed genes between postnatal day 1 and day 5 mouse ovaries using suppression subtractive hybridization(SSH) and found that MT transposon-like element, clone MTi7(MTi7) was one of the highly expressed genes in the day 5 mouse ovary(Park et al., 2002). Results of in situ hybridization and RNA interference revealed that the expression of MTi7 is oocyte-specific in the ovary and may be involved in the regulation of oocyte maturation(Park et al., 2003). At present, MTi7 sequence has been known only in the mouse. Therefore, the present study was accomplished 1) to identify MTi7 sequence in the other mammalian species, such as bovine, porcine, rat, and human, and 2) to evaluate the flanking sequence of the mouse MTi7 since it has transposon characteristics. Using ovarian cDNAs derived from four different species, we cloned and identified new MTi7 sequence showing a high degree of sequence homology with the mouse MTi7(87~98%). By using inverse PCR, we found that the mouse MTi7 may intercalated the beta-carotene 15, 15’-monooxygenase(Bcdo) gene and/or serine protease inhibitor, Kunitz type I(Spint 1) gene. By finding the MTi7 sequences in the other mammalian species and the flanking gene of the MTi7 in mouse, it is expected to reveal the role(s) of MTi7 in the oogenesis as well as folliculogenesis in the near future.
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KEYWORD
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